The IX53 has been designed from the ground up to be the finest microscope available for routine inverted microscopic analysis. The combination of excellent optical performance and mechanical quality results in a microscope system of outstanding value and comfort for regular use.
The IX53 system is the ideal solution for routine analysis. End-users can improve research efficiency by recording cell dynamics across a wide area. Reliability can also be enhanced through accurate position and light reproducibility.
IX53: One-deck System
An outstanding, cost-effective microscope for brightfield and fluorescence applications.
Olympus UIS2 infinity-corrected optics ensure high optical transmittance with a broad range of objectives. UIS2 optics feature wide chromatic correction and enable high resolution, high S/N primary images regardless of the observation method. The wide field of view and Fly-Eye lens system provide uniform fluorescence images and enable the use of sCMOS cameras with large sensors.
Apochromatic Objectives Enable High-Resolution Phase Contrast and Fluorescence Observation
Phase contrast apochromatic objectives (UPLSAPO100XOPH, PLAPON60XOPH) enable high-precision imaging free from image shift, even during simultaneous phase contrast and fluorescence observation. This eliminates the need to change objectives when switching observation methods.
Silicone Objectives* Enable High-Resolution Observation Deep in o Live Cells
Olympus offers three high-NA silicone immersion objectives:UPLSAPO30XS, UPLSAPO40XS, and UPLSAPO60XS. The refractive index of silicone oil (Refractive index: ne≈1.40) is close to that of living tissue (Refractive index: ne≈1.38), enabling high-resolution observation deep inside living tissue with minimal spherical aberration caused by refractive index mismatch. Silicone oil does not dry out or harden, so there is never a need to readminister the oil, making it ideal for extended time-lapse observations.
*Use dedicated silicone oil.
Special Objective Available for iPS/ES and Floating Cell Observation
This high-NA phase contrast objective (UCPLFLN20XPH) is especially suited for observation using plastic dishes. It enables high-resolution observation of the cell proliferation process and delivers improved contrast across a wide area.
High S/N Fluorescence Mirror Units for Efficient Signal Detection
All fluorescence mirror units feature filters treated with a specially-developed coating that minimizes noise by absorbing more than 99 % of stray light. The outstanding performance and high transmittance of the mirror units ensure efficient fluorescence signal detection.
Smooth Tracking at High Magnification
The IX3-SVR manual stage features a smooth positioning system which enables the easy tracking of cells, even at high magnifications. The observation position limits can be set by the user to immobilize the stage, ensuring that the observation position is maintained during operations such as reagent application, even if the stage is inadvertently touched. It is also possible to remove 35 mm dishes from the stage, place them in an incubator and return them to the exactly previously identified.
This feature was based on the technical development at the RIKEN BSI-Olympus Collaboration Center.
Easily Achieve Koehler Illumination
The condenser can be moved and easily reset to Koehler Illumination using the conveniently-located condenser lock and control knobs.
Long Life 130W Mercury Lamp (U-HGLGPS)
The U-HGLGPS fluorescence light source delivers bright, even illumination and features a long life, 2,000-hour mercury burner (average lifetime). The unit is maintenance-free and does not require centering adjustment. A liquid light guide prevents heat transfer to samples, thus allowing extended observations to be carried out without concern.
